Dies ist eine Übersichtsseite mit Metadaten zu dieser wissenschaftlichen Arbeit. Der vollständige Artikel ist beim Verlag verfügbar.
A rapid method for the isolation of functional thymus‐derived murine lymphocytes
4.166
Zitationen
3
Autoren
1973
Jahr
Abstract
Abstract A rapid method is described for effectively removing immunoglobulin‐bearing cells from either primed or unprimed mouse spleen and lymph node cell suspensions. Incubation of cell suspensions in nylon wool columns for 45 min at 37 °C resulted in a 9 to 100‐fold depletion of immunoglobulin‐bearing cells and a complementary 1.5 to 2‐fold enrichment of T cells in the column effluent populations. The effluent population, derived from passage of spleen cells through these columns, was virtually devoid of B precursor and memory cell activity, but contained all of the helper cell and cytotoxic effector cell precursor activity when compared to unfractionated spleen cells.
Ähnliche Arbeiten
SYRCLE’s risk of bias tool for animal studies
2014 · 3.958 Zit.
Histopathologic Technic and Practical Histochemistry.
1954 · 2.981 Zit.
Some new, simple and efficient stereological methods and their use in pathological research and diagnosis
1988 · 2.944 Zit.
Microdroplet Assay of Human Serum Cytotoxins
1964 · 2.076 Zit.
The methodological quality assessment tools for preclinical and clinical studies, systematic review and meta‐analysis, and clinical practice guideline: a systematic review
2015 · 2.067 Zit.