Dies ist eine Übersichtsseite mit Metadaten zu dieser wissenschaftlichen Arbeit. Der vollständige Artikel ist beim Verlag verfügbar.
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
48.724
Zitationen
2
Autoren
1959
Jahr
Abstract
Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.
Ähnliche Arbeiten
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
1959 · 22.742 Zit.
A PGC1-α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis
2012 · 4.613 Zit.
Metabolism of Isolated Fat Cells
1964 · 4.423 Zit.
The importance of the ratio of omega-6/omega-3 essential fatty acids
2002 · 4.065 Zit.
Dietary supplementation with n-3 polyunsaturated fatty acids and vitamin E after myocardial infarction: results of the GISSI-Prevenzione trial
1999 · 3.810 Zit.