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A novel method for tissue segmentation in high-resolution H&E-stained histopathological whole-slide images
55
Zitationen
3
Autoren
2019
Jahr
Abstract
Tissue segmentation in whole-slide images is an important task in digital pathology, required for efficient and accurate computer-aided diagnostics. Precise tissue segmentation is particularly significant for a correct diagnosis in cases, when tissue structure of a specimen is very porous, such as skin specimens. In this paper, we addressed the problem of fore- and background segmentation in histopatological images of skin specimens stained with hematoxylin and eosin (H&E), which has not been solved yet, by a novel method based on a combination of statistical analysis, color thresholding, and binary morphology. We validated our algorithm on large extracts from 60 high-resolution whole slide images, with differing staining quality and captured under varying imaging conditions, from three laboratories. The size of extracts varies from 2000×1500 to 20000×30000 pixels and the number of images used in our study matches the number of H&E images used by other research teams. We compared our method to the published ones (GrabCut and FESI) and showed that our approach outperforms its counterparts (Jaccard index of 0.929 vs. 0.776 and 0.695).
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